LOCUS       40924_23089       12333 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB480omega DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Nakagawa T, Tanaka Y, Kimura T, Ishiguro S.
  TITLE     Gateway binary vector pGWB for RNAi
  JOURNAL   Published Only in Database (2012)
REFERENCE   2  (bases 1 to 12333)
  AUTHORS   Nakagawa T, Tanaka Y, Kimura T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12333)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1091..1157
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1187..1311
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1336..1366
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1718..2020
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2064..2188)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2211..2565
                     /label=ICDH 1st intron (AT1G65930)
                     /note="ICDH 1st intron (AT1G65930)"
     protein_bind    2586..2710
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2754..3056)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3413..3537)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3571..3823
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3857..3873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3881..3897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3905..3935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3950..3971)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4005..4257)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4650..5438)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5462..5645)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5851..5875)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6403..7191
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7438..8026
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8212..8352)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8696..8890)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8959..10023)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10460..11086)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gtactcgaga tcctctagag ttatcaacaa gtttgtacaa
     1201 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1261 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
     1321 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     1381 taggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     1441 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     1501 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     1561 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     1621 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     1681 aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta
     1741 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     1801 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     1861 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     1921 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     1981 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     2041 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     2101 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     2161 ctcgttcagc tttcttgtac aaagtggttg ataacagcgc ttaactagtg gtgagatctt
     2221 acttctcctt ctcctctctc gatctctttc gctatctata atcagatatt ttcgttgatt
     2281 ggatacgaaa tgatttacct tcgcttgcct agattgtaac gaagtttcgt tgtttgttct
     2341 gatctgatct ctggaatcta cgcatgtgta aactcgttga ttgatttggt tttggtgtat
     2401 gattacgtta tagatcgata aaatcgataa aacctttaag ctctgttcat gagattgatt
     2461 gatttatgaa aaatctggac tcgatttcaa gtgtggattt gatttacagc tttgattcgg
     2521 cgaaatagtt tcgatattta agcttcattg gtttgtgtaa tgtaggtacc taagcgctgt
     2581 tatcaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata
     2641 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     2701 agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
     2761 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     2821 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     2881 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
     2941 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
     3001 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     3061 ccagtccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
     3121 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
     3181 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
     3241 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt tttgacatac
     3301 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
     3361 actgttatct ggcttttagt aagccggatc cagatctcga tcgaggggcc gccatagtga
     3421 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     3481 tatattgata tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgttga
     3541 taactctaga ggatgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     3601 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     3661 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     3721 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     3781 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     3841 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3961 aactcacatt aggctgaatt aggcgcgcct atttctgaat tcccgatcta gtaacataga
     4021 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
     4081 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
     4141 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
     4201 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
     4261 gatcatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg
     4321 gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgggccc
     4381 gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg gccgttgctg tcgtaatgat
     4441 atcggcacct tcgaccgcct gttccgcaga gatcccgtgg gcgaagaact ccagcatgag
     4501 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4561 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4621 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4681 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4741 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     4801 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4861 ggcaagcagg catcgccatg ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg
     4921 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4981 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5041 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5101 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5161 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5221 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     5281 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5341 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5401 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccagatccg
     5461 gtgcagatta tttggattga gagtgaatat gagactctaa ttggataccg aggggaattt
     5521 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
     5581 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
     5641 aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
     5701 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatg
     5761 aattcagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa
     5821 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     5881 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     5941 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6001 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6061 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     6121 cacgaaccca gtggacataa gcctcgttcg gttcgtaagc tgtaatgcaa gtagcgtaac
     6181 tgccgtcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     6241 cggttttcat ggcttcttgt tatgacatgt ttttttgggg tacagtctat gcctcgggca
     6301 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6361 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     6421 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     6481 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     6541 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     6601 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     6661 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     6721 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     6781 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     6841 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     6901 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     6961 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7021 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7081 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7141 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7201 gctagaaatt cgttcaagcc gacgccgctt cgccggcgtt aactcaagcg attagatgca
     7261 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     7321 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     7381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7561 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8221 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8281 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8341 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8401 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8461 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     8521 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     8581 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8641 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8701 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     8761 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     8821 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     8881 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     8941 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9001 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9061 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9121 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9181 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9241 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9301 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9361 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     9421 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     9481 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     9541 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9601 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9661 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9721 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     9781 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     9841 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     9901 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     9961 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10021 accgtcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10081 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10141 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10201 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10261 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10321 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10381 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10441 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10501 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    10561 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    10621 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    10681 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    10741 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    10801 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10861 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10921 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10981 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11041 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11101 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11161 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11221 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11281 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11341 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11401 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11461 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    11521 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    11581 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    11641 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    11701 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    11761 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    11821 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11881 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11941 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12001 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12061 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12121 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12181 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12241 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    12301 catgcacata caaatggacg aacggataaa cct
//
